LONZA人支气管上皮细胞 人原代细胞

LONZA人支气管上皮细胞

人原代细胞——人支气管上皮细胞英文说明：

中文名称：正常人支气管/气管上皮细胞
英文名称：NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid
货号：CC-2540
货期：现货
Cryopreserved Normal Human Bronchial Epithelial (NHBE) cells with Retinoic Acid
Product Overview

Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza's Normal Human Bronchial Epithelial Cells (NHBE) are available with retinoic acid. 

All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysi is provided for each cell lot purchased.

Select Applications with Lonza's Human Bronchial Epithelial Cells -

A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid intece (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid intece with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).

Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.

Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.

Purchase our Bronchial Epithelial Cell Culture Kit (catalog no. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog no. CC-2540) and BEGM^TMTM BulletKit^TM Growth Media (catalog no. CC-3170)

中文名称：正常人支气管/气管上皮细胞
英文名称：NHBE – Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture
货号：CC-2540S
货期：1个月
Cryopreserved Normal Human Bronchial Epithelial (NHBE) cells, screened and guaranteed for differentiation, in B-ALI^TM (Bronchial Air Liquid Intece) cell culture. 
Product Overview
Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs.  NHBEs labeled CC-2540S are suitable for air-liquid intece studies and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels.               
All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysi is provided for each cell lot purchased.

Select Applications with Lonza's Human Bronchial Epithelial Cells 

A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid intece (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid intece with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).

LONZA人支气管上皮细胞说明：